Biochemistry

• CaM binding
• Inducible activation technology ARIAD
• RIPA_lysates_of_cells
• Stripping buffer

Cell Culture

• Amaxa poration
• Banker culture of primary neurons Nat. Prot.
• Culture of PC12 cells
• Freezing cells
• Lipofectamine2000 for PC12 transfection
• Neuronal maintenance media
• PolyLysine coverslip preparation

Immunocytochemistry

• Immunofluorescence of neurons

Molecular Biology

• Antibiotics Preparation-Elisabeth
• Bacterial stocks
• LB Broth and Agar-Elisabeth
• Ligation
• Transformation